Coding

Part:BBa_K2114002:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-06)


aGFPnano_HA_G4S_cotZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of a HA epitope tag and a flexible GGGGS linker by primer extensions.


Source

The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies.

References